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KMID : 0380619870190040324
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Korean Journal of Food Science and Technology
1987 Volume.19 No. 4 p.324 ~ p.330
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Isolation of Methanol - assimilating Candida boidinii YF - 3 and Production of Single Cell Protein
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Abstract
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A large number of methanol-assimilating yeasts and bacteria were isolated from samples of soil, sewage, decomposed milk and spoiled sweet-radish pickles. Among the yeasts, one strain was selected and identified as a strain of Candida boidinii. In 1% (v/v) methanol Candida boidinii YF-3 grew well and could grow in as much as 5%. This yeast required boitin for growth. Maximum growth was observed at 30℃ and pH 6 in a semisynthetic medium. The productivity was 2.72g of dry cells per liter in batch culture with 1%(v/v) methanol and the cell yield for methanol was 0.39 gg^(-1). The specific growth rate was 0.11 h^(-1) and the generation time was 6.4 hours. The protein content of the cell was 45.5% and total nucleic acid content was 5.9%. The amino acid profile was as good as FAO standard for food protein.
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